Plasmid_Backbone

Part:BBa_K106697:Design

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
    Illegal NotI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
    Illegal BamHI site found at 5
    Illegal XhoI site found at 1086
    Illegal XhoI site found at 6550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2380
    Illegal NgoMIV site found at 697
    Illegal AgeI site found at 1993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4930
    Illegal SapI.rc site found at 3908
    Illegal SapI.rc site found at 6012


Design Notes

None.


Source

Variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)

References